Journal: Stem Cell Research & Therapy

Article Title: Human umbilical cord mesenchymal stem cell-derived exosomal miR-27b attenuates subretinal fibrosis via suppressing epithelial–mesenchymal transition by targeting HOXC6

doi: 10.1186/s13287-020-02064-0

Figure Lengend Snippet: hucMSC-Exo treatment had less fibrosis development in vivo. a Study design. b The RPE–Bruch membrane complex was disrupted, the normal arrangement of the inner nuclear layer was lost and neovascular hide beneath the subretinal space 7 days after the laser burn. Compared with the PBS group, the number of collagen fibers reduced (blue) in the group treated with hucMSC-derived exosomes 35 days after laser photocoagulation. c Choroidal neovascularization (CNV; dyed with isolectin B4) and fibrosis (dyed with collagen type I) were recorded 7, 21, and 35 days after laser burn in the PBS and hucMSC-Exo groups. d Mean volume of the CNV and fibrosis 7, 21, and 35 days after laser burn

Article Snippet: Then, the RPE–choroid complexes were washed, blocked with 5% goat serum albumin and 0.3% Triton X-100, and then incubated with FITC-labeled isolectin-B4 (IB4) (Vector Laboratories, 1:100) (for evaluating CNV) and collagen type I antibody (ab34710, Abcam, dilution 1:100) (for evaluating subretinal fibrosis) at 4 °C overnight.

Techniques: In Vivo, Derivative Assay

Journal: Frontiers in Cardiovascular Medicine

Article Title: Empagliflozin inhibits coronary microvascular dysfunction and reduces cardiac pericyte loss in db/db mice

doi: 10.3389/fcvm.2022.995216

Figure Lengend Snippet: Effects of empagliflozin treatment on the cardiac microvasculature of db/db mice. (A) Representative transverse images of myocardial vasculature showing capillaries (IB4+, red). (B) Statistical analysis of the density of capillaries in the transverse sections of myocardial tissue between the db/m group, db/db group, and db/db + EMPA group. (C) Representative longitudinal images of myocardial vasculature showing capillaries (IB4+, red). (D) Statistical analysis of the diameter of capillaries in the longitudinal sections of myocardial tissue between the db/m group, db/db group, and db/db + EMPA group. Data are presented as the means ± SEM. P -values are corrected for multiple comparisons. IB4, isolectin B4; HPF, high power field.

Article Snippet: After washing with PBS (2 h * 3), the heart tissues were incubated in 200 μL staining buffer with secondary antibody conjugated to Alexa Fluor 647 (AB32733, Invitrogen, Carlsbad, CA, United States, 1:200) and isolectin B4 conjugated to FITC (L2895, Merck Millipore, Darmstadt, Germany, 1:20) for 3 days at 37°C with gentle shaking.

Techniques:

Journal: Frontiers in Cardiovascular Medicine

Article Title: Empagliflozin inhibits coronary microvascular dysfunction and reduces cardiac pericyte loss in db/db mice

doi: 10.3389/fcvm.2022.995216

Figure Lengend Snippet: Effects of empagliflozin treatment on pericytes in the hearts of db/db mice. (A) Representative myocardial sections showing pericytes (NG2+, green), capillaries (IB4+, red) and nuclei (DAPI+, blue). (B) Statistical analysis of pericyte number/HPF between the three groups. (C) Statistical analysis of pericyte coverage between the three groups, pericyte coverage = (NG2 + area/IB4 + area)/HPF*100%. Data are presented as the means ± SEM. P -values are corrected for multiple comparisons. NG2, neuroglial cell 2 chondroitin sulfate proteoglycan; IB4, isolectin B4; DAPI, 4′,6-diamidino-2-phenylindole; HPF, high power field.

Article Snippet: After washing with PBS (2 h * 3), the heart tissues were incubated in 200 μL staining buffer with secondary antibody conjugated to Alexa Fluor 647 (AB32733, Invitrogen, Carlsbad, CA, United States, 1:200) and isolectin B4 conjugated to FITC (L2895, Merck Millipore, Darmstadt, Germany, 1:20) for 3 days at 37°C with gentle shaking.

Techniques:

Journal: Angiogenesis

Article Title: Protein disulfide isomerase A1 as a novel redox sensor in VEGFR2 signaling and angiogenesis

doi: 10.1007/s10456-022-09852-7

Figure Lengend Snippet: PDIA1 is required for postnatal angiogenesis in vivo. (a) Retinal whole-mount staining with Isolectin B4 (IB4) antibody of P5 pups from WT (n = 7) or Pdia1+/− (n = 9) mice. Arrowheads show tip cell sprouting and filopodia. The images show whole retina structure, remodeling plexus (branching points), and angiogenic fronts (tip cells). The quantitative results show retina length from center, number of branching points, and number of tip cells and the percentage of vascular retinal area to total retinal area. Results were presented as mean ± SEM. *p < 0.05. (b) Growth factor-reduced Matrigel containing VEGF (50 ng/mL), bFGF (100 ng/mF), heparin (60 unit) was injected subcutaneously in WT (n = 4) or Pdia1+/− (n = 4) mice and then harvested for analysis after 3 days. The neovascularization in Matrigel was determined by staining with isolectin-B4 antibody. The images were representative from 3 to 4 mice and presented as mean ± SEM. *p < 0.05, vs WT. (c) Aortic ring sprouting assay was monitored on Matrigel for 5–7 days [upper panel; lenti-scramble shRNA (shCont) vs lenti-shPdia1 RNA. Lower panel; WT mice (n = 3–4) vs Pdia1+/− mice (n = 3–4)]. Numbers of sprouting branch from endothelial cells were counted and normalized by control shRNA or WT mice. The images were representative from at least three different experiments or different mice and presented as mean ± SEM. *p < 0.05, **p < 0.01 vs control or WT. ns, not significant

Article Snippet: To determine the capillary density, Matrigel sections were stained with anti-mouse isolectin B4 antibody (BD Biosciences) followed by biotinylated anti-mouse IgG antibody (Vector Laboratories) as described previously [ 28 ].

Techniques: In Vivo, Staining, Injection, shRNA